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sdaAB [2020-08-04 09:25:47]

L-serine deaminase

locus

BSU_15850

5.10

23.67 kDa

protein length

220 aa Sequence Blast

gene length

663 bp Sequence Blast

function

serine utilization

product

L-serine deaminase (beta chain)

essential

4.3.1.17

synonyms

yloW,sdaA

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]

Gene

Coordinates

1,658,242 → 1,658,904

The protein

Catalyzed reaction/ biological activity

L-serine --> NH₄⁺ + pyruvate (according to UniProt)

Protein family

iron-sulfur dependent L-serine dehydratase family (with [protein|8361D87307D2E8350945F592ABD24E2109E2E81A|SdaAA], according to UniProt)

[SW|Domains]

[SW|ACT domain] (aa 148-220) (according to UniProt)

[SW|Cofactors]

Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

genes

description

[Pubmed|22383849]

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Biological materials

Mutant

BKE15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG

BKK15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG

lacZ fusion

pGP2275 (in [SW|pAC5]) (GP2967), available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

References

22383849,22686449,24161940,32743959